Fig. 6
From: ZAR1 is a novel epigenetically inactivated tumour suppressor in lung cancer
ZAR1 functions as a tumour suppressor in cancer cell lines through cell cycle arrest. a, b Localisation of overexpressed ZAR1 is exemplarily shown in A549 and HeLa with DAPI nuclear stain (×63 magnification). c–e Flow cytometry analysis found that overexpressed ZAR1 arrests the cell cycle in HeLa and HCT116 cancer cells. ZAR1 was overexpressed in cancer cells, cells were ethanol-fixed after 24 h (HeLa) and 48 h (HCT116) and propidium iodide staining was used to determine the DNA content for flow cytometry. The cell cycle distribution of HeLa and HCT116 cells upon ZAR1 reexpression is quantified (c, e), and gating is exemplarily depicted in HCT116 (d)