Fig. 5
From: ZAR1 is a novel epigenetically inactivated tumour suppressor in lung cancer
ZAR1 inhibits colony formation of lung cancer cell lines. ZAR1 was overexpressed in lung cancer cell lines A549, A427 and HTB171 and ZAR1 inhibited colony formation. Cells were transfected with ZAR1-EGFP or EGFP-vector control. Colonies were selected for 3 weeks with G418 and then Giemsa stained (a, upper panel). The according ZAR1 overexpression was verified by semi-quantitative RT-PCR (a, lower panel). Inhibition of colony formation by ZAR1 was quantified and showed significant (Student’s t test) reduction in colony numbers (b)