Fig. 2
From: ZAR1 is a novel epigenetically inactivated tumour suppressor in lung cancer
ZAR1 promoter hypermethylation in lung cancer cell lines (SCLC). ZAR1 promoter methylation status was analysed by COBRA assay in various SCLC lung cancer cell lines. CpG island region from the ZAR1 promoter was amplified from bisulfite-treated DNA and digested with Taq1. Digestion products indicate ZAR1 promoter methylation. Digestion products were separated on 2% TBE agarose gel with 100 bp marker (m methylated, um unmethylated, pos. positive control, + TaqI-digested, − mock digested)