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Fig. 1 | Clinical Epigenetics

Fig. 1

From: ZAR1 is a novel epigenetically inactivated tumour suppressor in lung cancer

Fig. 1

ZAR1 expression pattern, missing expression in lung cancer and promoter hypermethylation in lung cancer cell lines. ZAR1 expression in lung cancer cell lines A427, A549, HTB171, H322, H358 and HeLa (cervix carcinoma) versus normal tissue panel (breast, kidney, liver, lung and heart) by RT-PCR and semi-/quantitative PCR were determined. a, b Expression levels were normalised to ß-ACTIN (ACTB) and to normal lung tissue (=100%). Promoter hypermethylation of lung cancer cell lines, HEK and HeLa cells by COBRA analysis c and pyrosequencing for quantification d are shown. PCR and digestion products were separated on 2% TBE agarose gel with 100 bp marker M (m methylated, pm partially methylated, pos. positive control, neg. negative control; + TaqI digested, − mock digested)

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