Table 1 Primers used

qRT-PCR

HDAC1

fw CCAAGTACCACAGCGATGAC

rev CTGGACAGTCCTCACCAACG

HDAC3

fw TTGAGTTCTGCTCGCGTTACA

rev CCCAGTTAATGGCAATATCACAGAT

HDAC6

fw TGGCTATTGCATGTTCAACCA

rev GTCGAAGGTGAACTGTGTTCCT

CDKN1A

fw GACACCACTGGAGGGTGACT

rev CCACATGGTCTTCCTCTGCT

DNMT1

fw GATGTGGCGTCTGTGAGGT

rev CCTTGCAGGCTTTACATTTCC

DNMT3A

fw CCTGAAGCCTCAAGAGCAGT

rev TGGTGTCCTTCTGTTCTTTGC

EZH2

fw ACTGGCGAAGAGCTGTTTTT

rev TTCGATGCCGACATACTTCA

GAPDH

fw AACGGGAAGCTTGTCATCAA

rev TGGACTCCACGACGTACTCA

Pyrosequencing

CDKN1A enhancer

fw AGGAGGGAAGTGTTTTTTTGTAGTA

rev biotinylated-ACAACTACTCACACCTCAACTAAC

sequencing primer:

fw primer was used as sequencing primer in addition to:

S1 GGGTAGTTAGGAGT

S2 TTTGGTTTTTTTGAG

Genomic coordinates (hg38) of spanned region: CHRO STRAND 6 +, START 36678496, END 36678730

CDKN1A promoter

fw GGTTGGAATAGTTTGTTTTTAAGGA

rev biotinylated-AAATAAAAAAAACCCTTACCCTTC

sequencing primer:

fw primer was used as sequencing primer in addition to:

S GCGCGTTGTAGGG

Genomic coordinates (hg38) of spanned region: CHRO STRAND 6 +, START 36679579, END 36679743

CDKN1A gene body

fw GGGTTTTGGTTTGTTTAAGTTTTATT

rev biotinylated-CCACATAATCTTCCTCTACTATCC

sequencing primer:

fw primer was used as sequencing primer

Genomic coordinates (hg38) of spanned region: CHRO STRAND 6 +, START 36684308, END 36684436

ChIP

CDKN1A enhancer

fw GAAGCATGTGACAATCAACAACT

rev AAGCATCTTGAGGCCAGAAT

Genomic coordinates (hg38) of spanned region: CHRO STRAND 6 +, START 36678037, END 36678143

CDKN1A promoter

fw ACTCCAGAAGCCCTCTCC

rev GGCTTCCTTGGGAACAAACT

Genomic coordinates (hg38) of spanned region: CHRO STRAND 6 +, START 36679513, END 36679607

CDKN1A gene body

fw GAGACCCTCTGGTAGGAAGA

rev GAGATACAAGGAAGGCCCTG

Genomic coordinates (hg38) of spanned region: CHRO STRAND 6 +, START 36683986, END 36684088