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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Histone deacetylase inhibitors potentiate photodynamic therapy in colon cancer cells marked by chromatin-mediated epigenetic regulation of CDKN1A

Fig. 5

Effect of HDACis ± HY-PDT on cell cycle regulation. HT-29 cell cycle profiles (by PI-based flow cytometry) were measured after a sequential treatment starting with HDACis (for 24 h) followed by activation with hypericin for 24 h (a) or 48 h (b), as indicated. Samples treated with drug-free vehicle solvents (<0.1% DMSO) were used as the reference control. The results are expressed as percentage of total cycling cells and represent the average ± SD of three independent experiments each done in singlets. Data was analyzed using one-way ANOVA with Tukey post hoc test. All conditions were compared to the reference control (*p < 0.05, **p < 0.01, ***p < 0.001), and the combined treatments were compared to HY-PDT alone (ǂp < 0.05, ǂǂp < 0.01, ǂǂǂp < 0.001) and to correspondingly equal concentrations of HDACis alone (▲p < 0.05, ▲▲p < 0.01, ▲▲▲p < 0.001)

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