Fig. 1
From: Pulmonary microRNA profiling: implications in upper lobe predominant lung disease
Secretion of inflammatory cytokines by regional alveolar macrophages in response to hypoxia. Alveolar macrophages obtained via bronchoalveolar lavage (right upper and lower lobes) were subject to in vitro hypoxic conditions (5% O2 for 60 min) in an in vivo2 300 hypoxic chamber (Baker Ruskinn). Cytokines IL-8 and TNF-α (pg/ml) were measured by ELISA from conditioned media. Basal levels of cytokines secreted from AM are <35 pg/ml in 21% O2, however a significant increase in cytokine secretion occurs when macrophages are oxygen stressed at 5% O2 (ANOVA Tukey-Kramer HSD P < 0.0001). *Hypoxia induced increase in cytokine levels increase in both lobes (P < 0.0001). **Cytokine secretion was quantitatively higher from upper lobe AM (IL-8: upper 2294.6 (+731.8) vs. lower 830.4 (+415.8) pg/ml) (P < 0.0001). (TNF-α: upper 2656.9 (+986.8) vs. lower 1031.1 (+464.1) pg/ml) (P < 0.0001). Results from three independent experiments are reported as the means ± SD