Aberrant DNA methylation and expression of SPDEF and FOXA2 in airway epithelium of patients with COPD

Clinical Epigenetics

Table 1 Characteristics of subjects and experimental design

Culture condition		Age (years)	Sex	Smoking status	Pack years	FEV1%pred	FEV1/FVC%	N (RNA)	N (DNA)
ALI with IL-13^a	COPD 1	56	M	Ex	30	31	29	5	5
	COPD 2	59	F	Ex	41	87	59
	COPD 3	60	F	Ex	20	33	38
	COPD 4	56	F	Ex	30	14	25
	COPD 5	61	F	Ex	26	21	28
	Non-COPD controls 1–6	NA	NA	NA	NA	NA	NA	6	6
ALI without IL-13^b	COPD 6	58	F	Ex	40	18	25	7	8
	COPD 7	61	F	Ex	35	20	25
	COPD 8	55	F	Ex	18	19	52
	COPD 9	49	M	Ex	11	20	22
	COPD 10	60	M	Ex	38	16	29
	COPD 11	48	M	Ex	25	12	23
	COPD 12	58	F	Ex	38	60	46
	COPD 13	63	M	Non	0	41	45
	COPD 14	53	M	Ex	30	25	25
	COPD 15	57	M	Ex	30	11	31
	COPD 16	57	F	Ex	45	23	24
	Non-COPD controls 11–17	NA	NA	NA	NA	NA	NA	7	7

Abbreviations: COPD chronic obstructive pulmonary disease, M male, F female, Ex ex-smoker, Non non-smoker, NA not available, FEV1%pred forced expiratory volume during the first second as percentage of predicted, FEV1/FVC% the ratio of FEV1 to FVC (forced vital capacity), ALI air-liquid interface culture, IL-13 interleukin 13, N (RNA) number of RNA samples in total, N (DNA) number of DNA samples in total
^aCells were cultured at ALI in the presence of IL-13 and harvested after 0, 14, 21 and 28 days
^bCells were cultured at ALI without IL-13 and harvested after 14 days

ISSN: 1868-7083