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Table 4 LINE1 methylation pyrosequencing assay for males
| Criteria for comparison | Groups (n) | Global methylation fraction (mean ± SD) | P value | |
|---|---|---|---|---|
| ANOVA | Tukey’s post hoc test (vs controls) | |||
| Controls (33) | 0.5091 ± 0.0463 | – | – | |
| Mode of egg fertilization | ICSI (19) | 0.4739 ± 0.0339 | 0.0057 | < 0.05 |
| Non ICSI (37) | 0.4809 ± 0.0434 | < 0.05 | ||
| Oxygen tension | 20%(31) | 0.4738 ± 0.0404 | 0.0044 | < 0.01 |
| 5%(25) | 0.4843 ± 0.0399 | ns | ||
| Type of embryo transfer | Fresh (45) | 0.4751 ± 0.0386 | 0.0033 | < 0.01 |
| Frozen (11) | 0.4924 ± 0.0457 | ns | ||
| Embryo transfer day | Day 3 (28) | 0.4779 ± 0.0435 | 0.0067 | < 0.05 |
| Day 5 (28) | 0.4791 ± 0.0375 | < 0.05 | ||
