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Table 4 LINE1 methylation pyrosequencing assay for males

From: Global DNA methylation levels are altered by modifiable clinical manipulations in assisted reproductive technologies

Criteria for comparison Groups (n) Global methylation fraction (mean ± SD) P value
ANOVA Tukey’s post hoc test (vs controls)
  Controls (33) 0.5091 ± 0.0463
Mode of egg fertilization ICSI (19) 0.4739 ± 0.0339 0.0057 < 0.05
Non ICSI (37) 0.4809 ± 0.0434 < 0.05
Oxygen tension 20%(31) 0.4738 ± 0.0404 0.0044 < 0.01
5%(25) 0.4843 ± 0.0399 ns
Type of embryo transfer Fresh (45) 0.4751 ± 0.0386 0.0033 < 0.01
Frozen (11) 0.4924 ± 0.0457 ns
Embryo transfer day Day 3 (28) 0.4779 ± 0.0435 0.0067 < 0.05
Day 5 (28) 0.4791 ± 0.0375 < 0.05
  1. ANOVA analysis of variance; SD standard deviation; ICSI intracytoplasmic sperm injection; ns non significant
  2. Values in italics denote significance