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Table 3 LINE1 methylation pyrosequencing assay

From: Global DNA methylation levels are altered by modifiable clinical manipulations in assisted reproductive technologies

Criteria for comparison Groups (n) Global methylation fraction (mean ± SD) P value
ANOVA Tukey’s post hoc test (vs controls)
  Controls (65) 0.4997 ± 0.0463
Total ART ART (126) 0.4789 ± 0.0459 0.0034 a
Mode of egg fertilization ICSI (39) 0.4732 ± 0.0322 0.0090 < 0.05
Non ICSI (87) 0.4815 ± 0.0508 < 0.05
Oxygen tension 20%(73) 0.4781 ± 0.0526 0.0135 < 0.05
5%(53) 0.4799 ± 0.0352 ns
Type of embryo transfer Fresh (90) 0.4784 ± 0.0487 0.0137 < 0.05
Frozen (36) 0.4800 ± 0.0385 ns
Embryo transfer day Day 3 (67) 0.4783 ± 0.0529 0.0137 < 0.05
Day 5 (59) 0.4796 ± 0.0463 < 0.05
  1. ANOVA analysis of variance; ART assisted reproductive technology; SD standard deviation; ICSI intracytoplasmic sperm injection; ns non significant
  2. aUnpaired two-tailed t test
  3. Values in italics denote significance