Global DNA methylation levels are altered by modifiable clinical manipulations in assisted reproductive technologies

Table 2 Luminometric methylation assay (LUMA)

Criteria for comparison	Groups (n)	Global methylation fraction (mean ± SD)	P value
Criteria for comparison	Groups (n)	Global methylation fraction (mean ± SD)	ANOVA	Tukey’s post hoc test (vs controls)
	Controls (77)	0.4827 ± 0.0723	–	–
Total ART	ART (182)	0.5182 ± 0.0670	–	0.0002 ^a
Mode of egg fertilization	ICSI (54)	0.5224 ± 0.0533	0.0009	< 0.01
Mode of egg fertilization	Non ICSI (127)	0.5158 ± 0.0720	0.0009	< 0.01
Oxygen tension	20%(123)	0.5176 ± 0.0689	0.0009	< 0.01
Oxygen tension	5%(59)	0.5195 ± 0.0634	0.0009	< 0.01
Type of embryo transfer	Fresh (128)	0.5109 ± 0.0684	< 0.0001	< 0.05
Type of embryo transfer	Frozen (54)	0.5357 ± 0.0608	< 0.0001	< 0.0001
Embryo transfer day	Day 3 (110)	0.5212 ± 0.0689	0.0007	< 0.001
Embryo transfer day	Day 5 (72)	0.5138 ± 0.0643	0.0007	< 0.05

ANOVA analysis of variance; ART assisted reproductive technology; SD standard deviation; ICSI intracytoplasmic sperm injection
^aUnpaired two-tailed t test
Values in italics denote significance

ISSN: 1868-7083