Fig. 5

Comparison of uniquely hypomethylated probe between human methylation 450K bead chip and TCGA samples. Methylation status of unique probes on a CD86 and b CTLA4 promoter in OSCC (disease) and adjacent normal samples (normal) in India and different stages of TCGA samples (S1, stage 1; S2, stage 2; S3, stage 3; and S4, stage 4A). Methylation profile of these genes in disease tissue of Indian patients was significantly different from their normal counterpart, as well as from disease stages S1, S2, S3, and S4A of TCGA samples. Gene expression (fold change) of CD86 and CTLA4 showed significantly higher expression in OSCC compared in the adjacent normal tissues among Indian samples. P value ≤0.05 was considered significant