Fig. 1

RRx-001 inhibited cell growth and DNMT1 and DNMT3a expression in HCT 116 cells. Exponentially growing cells were treated with 5-AZA, RRx-001 or 0.025% DMSO as a vehicle control for 1 to 3 days. Media containing RRx-001, 5-AZA, or DMSO were replaced daily over the 3-day period. a Concentration-survival curve of HCT 116 cells treated with RRx-001. SCC VII cells were used as a positive control. Data are mean ± SD from 3 independent experiments. b Protein levels of DNMT1 and DNMT3a in HCT 116 cells treated with RRx-001 as determined by Western blot. β-actin was used as an internal control for sample loading. 5-AZA was used as a positive control for inhibition of DNMT1 and DNMT3a expression. Bar graphs show the relative expression of DNMT1 and DNMT3a in treated cells compared to control cells