Fig. 3

a Heatmap showing differential miRNA expression in diseased vs normal control group. Green color indicates lower than mean intensity, and red represents higher than mean intensity. Each row represents a miRNA and each column represents a sample (p < 0.05, logFC > |1)|. b Comparison of tubular and tubulovillous adenoma with colorectal cancer tissue samples. Each row represents a miRNA. Each column represents a tissue sample. Selected miRNAs are sorted by statistical conditions: p< 0.05, logFC > |1|. c Comparison of tubular and tubulovillous adenoma samples. Each row represents a miRNA. Each column represents a tissue sample. Selected miRNAs are sorted by statistical conditions: p< 0.05, logFC > |1|. d Cluster analysis of Dukes B and D stages. Results based on three miRNAs showed significant expression alteration between groups (p < 0.05). e Real-time PCR (E/2) validation of microarray (E/1) results in fresh frozen biopsy tissue samples. N normal; AD tubular adenoma; AD _TV tubulovillous adenoma; CRC colorectal cancer