Table 1 Clinicopathological data of 71 biliary tract cancer patients included in the tissue study. Associations of clinicopathological data with relative DNA methylation of SHOX2 and SEPT9 in tumor tissue
| Â | Â | Â | SHOX2 methylation | SEPT9 methylation |
|---|---|---|---|---|
Clinicopathological data | n | (%) | ||
| Â | Â | Â | p value (n) | p value (n) |
Tumor location | ||||
 GBC | 17 | (24) |  |  |
 IHC | 25 | (35) |  |  |
 PHC (Klatskin tumor) | 22 | (31) |  |  |
 DC | 7 | (10) |  |  |
 GBC vs. CC |  |  | 0.46 (55) | 0.014* (55) |
Gender | ||||
 Male | 42 | (59) |  |  |
 Female | 29 | (41) |  |  |
 Male vs. female |  |  | 0.10 (55) | 0.14 (55) |
Age at diagnosis | ||||
 ≤60 years | 31 | (44) |  |  |
 >60 years | 40 | (56) |  |  |
 Median age [years] | 63 | NA |  |  |
 Mean age [years] | 62 | NA |  |  |
 Range [years] | 36–83 | NA |  |  |
 ≤60 vs. >60 years |  |  | 0.57 (55) | 0.031* (55) |
Tumor grade | ||||
 G1 | 5 | (7) |  |  |
 G2 | 33 | (46.5) |  |  |
 G3 | 33 | (46.5) |  |  |
 G1, G2, vs. G3 |  |  | 0.22 (55) | 0.68 (55) |
Tumor stage | ||||
 T1 | 10 | (14) |  |  |
 T2 | 17 | (24) |  |  |
 T3 | 38 | (53.5) |  |  |
 T4 | 6 | (8.5) |  |  |
 T1, T2 vs. T3, T4 |  |  | 0.51 (55) | 0.013* (55) |
Lymph node status | ||||
 N0 | 32 | (45.1) |  |  |
 N1 | 15 | (21.1) |  |  |
 N2 | 1 | (1.4) |  |  |
 Unknown | 23 | (32.4) |  |  |
 N0 vs. N1, N2 |  |  | 0.19 (39) | 0.30 (39) |
Venous invasion | ||||
 V0 | 42 | (59) |  |  |
 V1 | 19 | (27) |  |  |
 Unknown | 10 | (14) |  |  |
 V0 vs. V1 |  |  | 0.97 (48) | 0.76 (48) |
Lymphatic invasion | ||||
 L0 | 34 | (48) |  |  |
 L1 | 20 | (28) |  |  |
 Unknown | 17 | (24) |  |  |
 L0 vs. L1 |  |  | 0.67 (42) | 0.70 (42) |
Perineural invasion | ||||
 Pn0 | 17 | (24) |  |  |
 Pn1 | 35 | (49) |  |  |
 Unknown | 19 | (27) |  |  |
 Pn0 vs. Pn1 |  |  | 0.37 (41) | 0.82 (41) |
Distant metastases | ||||
 M0 | 65 | (91.5) |  |  |
 M1 | 6 | (8.5) |  |  |
 M0 vs. M1 |  |  | 0.32 (55) | 0.36 (55) |
UICC stage | ||||
 UICC I | 4 | (6) |  |  |
 UICC II | 9 | (13) |  |  |
 UICC III | 28 | (39) |  |  |
 UICC IV | 10 | (14) |  |  |
 Unknown | 20 | (28) |  |  |
 UICC I, II vs. UICC III, IV |  |  | 0.08 (41) | 0.06 (41) |
Surgical margin | ||||
 R0 | 52 | (73) |  |  |
 R1 | 19 | (27) |  |  |
 R0 vs. R1 |  |  | 0.24 (55) | 0.66 (55) |
Follow-up | ||||
 Follow-up available | 71 | (100) |  |  |
 Median follow-up [months] | 15 | NA |  |  |
 Mean follow-up [months] | 23 | NA |  |  |
 Range [months] | 0–104 | NA |  |  |
 Deceased | 50 | (70) |  |  |
 Censored | 21 | (30) |  |  |