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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Assessment of global DNA methylation in the first trimester fetal tissues exposed to maternal cigarette smoking

Fig. 2

Global DNA methylation in fetal livers and small intestines. a DNA methylation in the fetal liver was determined by ELISA using 5-mC-specific antibody (non-smoking group N = 10 and smoking group N = 11). Data represent the means of three experiments. b–c Percentage of methylation of AluYb8 and LINE-1 in the fetal liver was determined by pyrosequencing bisulfite-converted DNA (non-smoking group N = 15 and smoking group N = 18). d DNA methylation in the fetal small intestine was determined by ELISA using 5-mC-specific antibody (non-smoking group N = 7 and smoking group N = 12). Data represent the means of 2 experiments. e–f Percentage of methylation of AluYb8 (non-smoking group N = 9 and smoking group N = 12) and LINE-1 (non-smoking group N = 9 and smoking group N = 11) was determined by pyrosequencing bisulfite-converted DNA. Percentages of methylation were calculated, analyzed and displayed as described in the legend for Fig. 1

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Clinical Epigenetics

ISSN: 1868-7083

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