Fig. 5

Methylation status of promoter regions of SOX21 and SOX21-AS1 in 86 patients with OSCC. a Methylation status of three CpG-rich regions of the SOX21-AS1 promoter was analyzed in the OSCC genome by using COBRA. Arrows indicate the unmethylated (u)/methylated (m) alleles. We quantified the DNA fragments by using ImageJ, and the DNA methylation status was calculated according to the digested fragment/(undigested fragment + digested fragment) (lower panels). b Methylation status of one patient (patient 9) examined using bisulfite sequencing. Each row represents a single clone for each PCR product. Open and filled squares represent unmethylated and methylated CpG sites, respectively