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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Aberrant DNA hypermethylation-silenced SOX21-AS1 gene expression and its clinical importance in oral cancer

Fig. 3

Promoter activity of SOX21-AS1 was silenced using in vitro methylation. a Schema of the luciferase constructs containing the putative promoter of SOX21-AS1 (pGL4.21-SOX21-AS1-P 0–829 bp). b Promoter constructs were transfected into SAS cells for 24 h, and luciferase activity was examined using the Dual-Glo luciferase reporter assay system kit. The SV40 promoter was the positive control. Relative luciferase activity was compared using the ratio of Renilla reniformis and firefly luciferase activity. c pGL4.21-SOX21-AS1-P was methylated in vitro by using M. SssI, M. HpaII, and M. HhaI methylase enzymes. The promoter activity was examined using the luciferase reporter assay

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