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Table 2 Primers for PCR amplification and pyrosequencing

From: Variations in DNA methylation of interferon gamma and programmed death 1 in allograft rejection after kidney transplantation

Gene Primers CpGs
IFNγ F: 5′-ATGGTATAGGTGGGTATAATGG-3′  
R: 5′-biotin-CAATATACTACACCTCCTCTAACTAC-3′  
S: 5′-GGTGGGTATAATGGG-3′ CpG-186
S: 5′-ATTATTTTATTTTAAAAAATTTGTG-3′ CpG-54
PD1 F: 5′-AGTATAGAATATAAGGAGATAAGTAAGT-3′  
R: 5′-biotin-CCATAACCACAATTCCAAATCTTT-3′  
S: 5′-AGAATATAAGGAGATAAGTAAGTT’-3′ CpG-914, CpG-911, CpG-906
S: 5′-GGATTTTTTGAATTATTTTATTTTG′-3′ CpG-857, CpG-833
S: 5′-TTAGTTTTATAGTTAGTTTTTG-3′ CpG-776, CpG-762, CpG-738
  1. F forward primer, R reverse primer, S sequencing primer, CpGs cytosine phosphate guanine sites