Table 2 Primers for PCR amplification and pyrosequencing
Gene | Primers | CpGs |
|---|---|---|
IFNγ | F: 5′-ATGGTATAGGTGGGTATAATGG-3′ | |
R: 5′-biotin-CAATATACTACACCTCCTCTAACTAC-3′ | ||
S: 5′-GGTGGGTATAATGGG-3′ | CpG-186 | |
S: 5′-ATTATTTTATTTTAAAAAATTTGTG-3′ | CpG-54 | |
PD1 | F: 5′-AGTATAGAATATAAGGAGATAAGTAAGT-3′ | |
R: 5′-biotin-CCATAACCACAATTCCAAATCTTT-3′ | ||
S: 5′-AGAATATAAGGAGATAAGTAAGTT’-3′ | CpG-914, CpG-911, CpG-906 | |
S: 5′-GGATTTTTTGAATTATTTTATTTTG′-3′ | CpG-857, CpG-833 | |
S: 5′-TTAGTTTTATAGTTAGTTTTTG-3′ | CpG-776, CpG-762, CpG-738 |