Table 3 Overview of the number of cells required for the various epigenetic profiling assays
From: Genome-wide epigenomic profiling for biomarker discovery
Epigenomic profiling method | Cell input using traditional profiling on bulk cells to obtain optimal data quality | Cell input using miniaturized profiling | Compatible with single-cell readout | Compatible with single cell as input |
|---|---|---|---|---|
WGBS | 3 × 103 | 3 × 103 | ✓ | ✓ |
ChIP-Seq | 0.5–5 × 106* | 1 × 104 or more | ✓ | |
DNAseI-Seq | 1–10 × 106 | 1 × 103 | ✓ | ✓ |
ATAC-Seq | 5 × 104 | 2 × 102 | ✓ | ✓ |
Hi-C-Seq | 2.5 × 107 | 1 × 107 | ✓ | |
MNase-Seq | 1 × 106 | – |