Fig. 7
From: Maternal vitamin D depletion alters DNA methylation at imprinted loci in multiple generations
Diet-dependent DNA methylation profile in liver and sperm of G1 and G2. a Heat maps of difference in methylation (value = median of LVD methylation—median of CON methylation) detected in comparing LVD vs. CON groups. Negative values indicate hypomethylation in LVD group and positive values indicate hypermethylation in LVD group. Values represented by shades of color indicated below each panel. b Principal component analysis (PCA) plots for cross 1 and cross 2 was performed for each cell type, generation, and developmental timepoint using difference in methylation at all 7 loci (Igf2DMR1, H19Cbs2, H19Cbs4, IG-DMR, SnrpnICR, and Grb10DMR) between medians of LVD and CON groups. Principal component 1(PC1) and PC2 are the principal components that explain the majority of the variation and are represented on the x-axis and y-axis, respectively. Percent variation explained by each component is listed in parentheses on each axis