Fig. 6
From: Maternal vitamin D depletion alters DNA methylation at imprinted loci in multiple generations
G2 male neonatal DNA methylation and gene expression patterns. a Bar graphs depict average DNA methylation across samples in the respective treatment group assayed in neonatal G2 liver (in order from left to right, N = 10 for all loci except H19PP N = 10, 9, 10, 10). For each sample, the average methylation for all CpGs assayed across the locus is represented as a dot within the bar graph (see the “Methods” section). b, c mRNA abundance relative to housekeeping genes Rplp0 and Gapdh (N = 10 for all genes). DMR differentially methylated region, ICR imprinted control region. Median values are indicated and linked by a horizontal line between box and whisker plots. Asterisks (*) indicates p value <0.05 determined by t test within each cross; panel above each graph lists all statistically significant (p value <0.05) comparisons determined by regression analysis for overall diet-dependent effects (diet), diet-independent grandparent of origin effects (GPO), and diet-dependent grandparent of origin effects (diet × GPO). n.s. not significant