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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Maternal vitamin D depletion alters DNA methylation at imprinted loci in multiple generations

Fig. 1

Experimental crosses and dietary treatment scheme. a Dietary treatments are indicated in middle block: CON (AIN-93G), LVD (lacking vitamin D), standard chow (Teklad 8604). Arrows above indicate treatment at developmental timepoints. Shaded regions below dietary treatment scheme indicate treatment windows and time of harvest (dashed vertical line). b Illustration of crosses used in paternal transmission of genomes exposed to vitamin D depletion (highlighted region depicts direct exposure), developmental timepoints and cell types tested (parentheses). c Maternal (G0 dam) plasma 25(OH)D levels (N = 5, 3, 5, 6, in order from left to right). X-axis indicates dam strain. G 0 parental generation 0, G 1 first generation of offspring, G 2 second generation of offspring, PND postnatal day. Different letters on bars represent statistically significant groups with p value <0.05. Panel above the graph lists all statistically significant (p value <0.05) comparisons determined by regression analysis for overall diet-dependent effects (diet) and diet × strain interactive effects (diet × strain)

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