Fig. 6

Differential binding of H3K4me3 at gene promoter sites during monocyte development results in changes in gene and protein expression. a The presence of H3K4me3 peaks around the promoter site of CCR2 in adult monocytes without an appreciable H3K4me3 peak in term neonatal monocytes is associated with significantly more CCR2 mRNA expression and CCR2 surface staining by flow cytometry in unstimulated adult monocytes. b The presence of H3K4me3 peaks around the promoter site of CD300C in adult monocytes without an appreciable H3K4me3 peak in term neonatal monocytes is associated with significantly more CD300C mRNA expression and CD300C surface staining by flow cytometry in adult monocytes only after LPS 100 ng/mL in vitro stimulation. c Increased H3K4me3 peaks around the promoter site of ILF2 in adult monocytes are associated with significantly more ILF2 mRNA expression in unstimulated adult monocytes. d Comparable amounts of H3K4me3 peaks around the promoter site of CXCR4 in adult and term neonatal monocytes are associated with comparable CXCR4 mRNA expression and CXCR4 surface staining by flow cytometry in unstimulated monocytes. A = adult, T = term. H3K4me3 peaks display the read coverage for H3K4me3 from one of the representative replicates from each group. Black bars display the H3K4me3 consensus peaks after only peaks present in at least two replicates are combined and analyzed. For the mRNA studies, adult n = 9, term n = 9. For the flow cytometry studies, adult n = 4, term n = 5. *p < 0.05, **p < 0.01