Fig. 1

H3S10ph level in paired tumors and negative PRM and DRM GC tissue samples: a immunoblot analysis of H3S10ph, H4K16ac, and H4K20me3 in freshly resected paired tumor and resection margin tissues (n = 10). b Representative image (left panel, ×40) and comparison of mean H-score (right panel) for H4K16ac and H4K20me3 immunostaining (n = 10). c Representative images (×40) of H3S10ph immunostaining (n = 101). d Comparison of frequency distribution with low (H-score 0–100), intermediate (H-score 100–200), and high (H-score 200–300) levels of H3S10ph immunostaining (n = 101). e Comparative mean H-score of H3S10ph immunostaining (n = 101). GC gastric cancer, PRM proximal resection margin, DRM distal resection margin, RM resection margin either PRM or DRM with maximum distance from the site of the tumor, P patient. Statistical tests are done by using Wilcoxon matched pairs test. *p < 0.05, **p < 0.01, ***p<0.001