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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Histone modification signature at myeloperoxidase and proteinase 3 in patients with anti-neutrophil cytoplasmic autoantibody-associated vasculitis

Fig. 5

ChIP-quantitative PCR analysis for histone modifications at autoantigen genes in ANCA-patients with high and low PRTN3 and MPO expression. a The level of H3K9me2 is reported as relative percent input for AAV patients with active disease (BVAS ≥ 3) and high expression of PRTN3 and MPO (↑mRNA, black triangles, n = 7) and for healthy controls (HC; light gray triangles, n = 21) at the PRTN3 promoter (↑mRNA 3.41 ± 5.20, HC 21.17 ± 28.55; p = 0.0068) and the MPO promoter (↑mRNA 3.34 ± 6.02, HC 20.01 ± 26.00; p = 0.0058). The level of H3K9me2 is reported as relative percent input for AAV patients in remission (BVAS = 0) and low expression of PRTN3 and MPO (↓mRNA, gray triangles, n = 8) for healthy controls at the PRTN3 promoter (↓mRNA 20.33 ± 28.30, HC 21.17 ± 28.55; p = 0.294) and at the MPO promoter (↓mRNA 18.41 ± 23.88, HC 20.01 ± 26.00; p = 0.393). b The level of H4K16ac is reported as relative percent input for AAV patients with active disease (BVAS ≥ 3) and high expression of PRTN3 and MPO (↑mRNA, black triangles, n = 17) and for healthy controls (HC; light gray triangles, n = 20) at the PRTN3 promoter (↑mRNA 19.12 ± 9.31, HC 10.83 ± 2.73; p = 0.0009) and the MPO promoter (↑mRNA 33.26 ± 11.03, HC 21.56 ± 6.67; p = 0.0006). The level of H4K16ac is reported as relative percent input for AAV patients in remission (BVAS = 0) and low expression of PRTN3 and MPO (↓mRNA, gray triangles, n = 8) for healthy controls at the PRTN3 promoter (↓mRNA 12.81 ± 7.55, HC 10.83 ± 2.73; p = 0.939) and at the MPO promoter (↓mRNA 24.02 ± 15.02, HC 21.56 ± 6.67; p = 0.859). (Note: the level of the indicated histone modification was calculated using raw Ct values from qPCR of diluted input sample. The median expression value is represented by the line in the box of the box and whisker plot, while mean ± standard deviation is listed in figure legend.) c Illustration depicts a model for modifications of histone tails at promoters of PRTN3 and MPO in healthy controls and AAV patients in remission (top), and in AAV patients with active disease (bottom)

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