Fig. 4

Chromatin immunoprecipitation (ChIP)-quantitative PCR analysis for histone modifications at autoantigen genes in AAV patients and healthy controls. a ChIP-qPCR was performed on AAV patients (ANCA, black triangles, n = 15) and healthy controls (HC; gray triangles, n = 21) for H3K9me2. The level of the H3K9me2 modification is reported as relative percent of input at the PRTN3 promoter (ANCA 12.44 ± 22.10, HC 21.17 ± 28.55; p = 0.0192), MPO promoter (ANCA 11.37 ± 19.01, HC 20.01 ± 28.55; p = 0.0247), and a control gene, MYO-D (ANCA 21.42 ± 28.78, HC 23.63 ± 35.53; p = 0.702). b ChIP-qPCR for H4K16ac was performed on AAV patients (ANCA, black triangles, n = 25) and healthy controls (HC; gray triangles, n = 20). The level of the H4K16ac modification is reported as relative percent of input at the PRTN3 promoter (ANCA 17.10 ± 9.14, HC 10.83 ± 2.73; p = 0.0116), MPO promoter (ANCA 30.30 ± 12.89, HC 21.06 ± 6.67; p = 0.0132) and at a control gene, FCGR3B (ANCA 9.19 ± 4.74, HC 8.83 ± 5.14, p = 0.864). c ChIP-qPCR for H3K4me2 was performed on AAV patients (ANCA, black triangles, n = 8) and healthy controls (HC, gray triangles, n = 8). The level of the H3K4me2 modification is reported as relative percent of input at the PRTN3 promoter (ANCA 50.68 ± 18.15, HC 63.78 ± 32.86, p = 0.532), MPO promoter (ANCA 24.17 ± 14.80, HC 36.09 ± 18.88, p = 0.136), and at a control gene, FCGR3B (ANCA 74.01 ± 30.26, HC 82.74 ± 46.52, p = 0.878) (Note: the level of the indicated histone modification was calculated using raw Ct values from qPCR of diluted input sample. The median expression value is represented by the line in the box of the box and whisker plot, while mean ± standard deviation is listed in figure legend)