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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Histone modification signature at myeloperoxidase and proteinase 3 in patients with anti-neutrophil cytoplasmic autoantibody-associated vasculitis

Fig. 2

Quantitative RT-PCR analysis of candidate genes in leukocytes from AAV patients and health controls. a Quantitative RT-PCR was performed on RNA isolated from total leukocytes of AAV patients (n = 80, black ovals) and healthy controls (HC; n = 20, light gray ovals). Expression is reported as a relative fold change for EHMT1 (ANCA 0.51 ± 0.12 versus HC 0.69 ± 0.17, p < 0.0001), EHMT2 (ANCA 0.26 ± 0.17 versus HC 0.51 ± 0.35, p < 0.0001), MSL1 (ANCA 3.07 ± 1.07 versus HC 2.16 ± 0.89, p = 0.0009), and ING4 (ANCA 0.63 ± 0.14 versus HC 0.79 ± 0.14, p < 0.0001). b AAV patients were divided into two groups: (1) patients with active disease (BVAS ≥ 3) and high expression of autoantigen genes PRTN3 and MPO (↑mRNA, black ovals, n = 40), (2) patients in remission (BVAS = 0) and low expression of autoantigen genes PRTN3 and MPO (↓mRNA, gray ovals, n = 40). Expression comparing AAV patients with active disease and AAV patients in remission is reported as a relative fold change for EHMT1 (↑mRNA 0.47 ± 0.11 versus ↓mRNA 0.54 ± 0.11, p = 0.0172), EHMT2 (↑mRNA 0.22 ± 0.17 versus ↓mRNA 0.29 ± 0.15, p = 0.0074), MSL1 (↑mRNA 3.49 ± 1.07 versus ↓mRNA 2.64 ± 0.91, p = 0.0004), and ING4 (↑mRNA 0.57 ± 0.14 versus ↓mRNA 0.69 ± 0.11, p < 0.0001). (Note: the median expression value is represented by the line in the box of the box and whisker plot, while mean ± standard deviation is listed in figure legend)

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