Obesity-related DNA methylation at imprinted genes in human sperm: Results from the TIEGER study

Soubry, Adelheid; Guo, Lisa; Huang, Zhiqing; Hoyo, Cathrine; Romanus, Stephanie; Price, Thomas; Murphy, Susan K.

doi:10.1186/s13148-016-0217-2

Clinical Epigenetics

Table 3 Multivariate analyses: DNA methylation at the DMRs of imprinted genes in sperm cells of obese and overweight volunteers versus normal weight men

From: Obesity-related DNA methylation at imprinted genes in human sperm: Results from the TIEGER study

	Imprinted genes
CpG sites	MEG3-IG			MEG3			PEG1/MEST			IGF2			H19			GRB10
CpG sites	%	SE	p	%	SE	p	%	SE	p	%	SE	p	%	SE	p	%	SE	p
CpG 1				−1.43	0.62	0.02	−0.06	0.21	0.76	+0.94	0.41	0.02	+1.34	0.68	0.05	−0.60	0.35	0.09
CpG 2	+2.33	0.89	0.01	−2.01	0.88	0.03	+0.21	0.16	0.19	+0.18	0.72	0.81	+1.43	1.61	0.38	−0.38	0.29	0.20
CpG 3	+1.20	0.66	0.08	−2.12	0.92	0.02	+0.44	0.27	0.11	+0.27	0.66	0.69	+1.41	0.58	0.02	−0.70	0.31	0.03
CpG 4	+0.44	0.83	0.59	−1.99	0.84	0.02	+0.18	0.38	0.64				+1.31	0.51	0.01	−0.41	0.32	0.21
CpG 5	+0.89	0.76	0.24	−2.04	0.91	0.03										−0.42	0.29	0.15
CpG 6				−2.04	0.91	0.03										−0.50	0.28	0.08
CpG 7				−2.19	0.86	0.01
CpG 8				−1.99	0.86	0.02
CpG 9
CpG 10
Mean	+1.22	0.59	0.04	−1.99	0.84	0.02	+0.19	0.17	0.26	+0.46	0.52	0.38	+1.37	0.62	0.03	−0.50	0.29	0.09
CpG site	NNAT			NDN			SNRPN			SGCE/PEG10			PEG3			PLAGL1
CpG site	%	SE	p	%	SE	p	%	SE	p	%	SE	p	%	SE	p	%	SE	p
CpG 1	−0.71	0.58	0.22	−1.13	0.53	0.04	−0.60	0.31	0.05	−2.04	0.98	0.04	−0.73	0.40	0.07	−2.79	1.78	0.12
CpG 2	−0.67	0.47	0.16	−1.28	0.56	0.03	−0.65	0.28	0.02	−2.27	1.02	0.03	−0.82	0.44	0.07	−2.93	1.76	0.10
CpG 3	−0.49	0.55	0.37	−0.75	0.36	0.04	−0.76	0.33	0.02	−2.49	1.05	0.02	−0.66	0.45	0.14	−2.36	1.72	0.17
CpG 4				−1.13	0.53	0.04	−0.64	0.25	0.01	−2.61	0.97	0.01	−0.70	0.38	0.07	−3.13	1.74	0.08
CpG 5				−1.22	0.52	0.02				−2.78	1.02	0.01	−0.88	0.50	0.08	−2.63	1.67	0.12
CpG 6				−1.08	0.44	0.02				−2.85	1.07	0.01	−0.80	0.45	0.08	−2.10	1.54	0.18
CpG 7													−0.74	0.43	0.09
CpG 8													−0.61	0.39	0.12
CpG 9													−0.75	0.43	0.09
CpG 10													−0.62	0.39	0.12
Mean	−0.62	0.50	0.22	−1.10	0.47	0.02	−0.65	0.27	0.02	−2.51	1.01	0.01	−0.73	0.42	0.09	−2.66	1.67	0.12

The model shown adjusts for age (as a continuous variable) and being a patient (yes/no) at the fertility clinic as possible confounding variables. Percentages for each CpG site represent the deviation of methylation percentage for overweight/obese men (BMI ≥ 25) from the methylation percentage of normal weight men (BMI < 25). These deviations were also averaged over all CpG sites associated with a particular imprinted gene (shown on the bottom line).

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