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Table 3 Multivariate analyses: DNA methylation at the DMRs of imprinted genes in sperm cells of obese and overweight volunteers versus normal weight men

From: Obesity-related DNA methylation at imprinted genes in human sperm: Results from the TIEGER study

  Imprinted genes
CpG sites MEG3-IG MEG3 PEG1/MEST IGF2 H19 GRB10
% SE p % SE p % SE p % SE p % SE p % SE p
CpG 1   −1.43 0.62 0.02 −0.06 0.21 0.76 +0.94 0.41 0.02 +1.34 0.68 0.05 −0.60 0.35 0.09
CpG 2 +2.33 0.89 0.01 −2.01 0.88 0.03 +0.21 0.16 0.19 +0.18 0.72 0.81 +1.43 1.61 0.38 −0.38 0.29 0.20
CpG 3 +1.20 0.66 0.08 −2.12 0.92 0.02 +0.44 0.27 0.11 +0.27 0.66 0.69 +1.41 0.58 0.02 −0.70 0.31 0.03
CpG 4 +0.44 0.83 0.59 −1.99 0.84 0.02 +0.18 0.38 0.64   +1.31 0.51 0.01 −0.41 0.32 0.21
CpG 5 +0.89 0.76 0.24 −2.04 0.91 0.03    −0.42 0.29 0.15
CpG 6   −2.04 0.91 0.03 −0.50 0.28 0.08
CpG 7 −2.19 0.86 0.01  
CpG 8 −1.99 0.86 0.02
CpG 9  
CpG 10
Mean +1.22 0.59 0.04 −1.99 0.84 0.02 +0.19 0.17 0.26 +0.46 0.52 0.38 +1.37 0.62 0.03 −0.50 0.29 0.09
CpG site NNAT NDN SNRPN SGCE/PEG10 PEG3 PLAGL1
% SE p % SE p % SE p % SE p % SE p % SE p
CpG 1 −0.71 0.58 0.22 −1.13 0.53 0.04 −0.60 0.31 0.05 −2.04 0.98 0.04 −0.73 0.40 0.07 −2.79 1.78 0.12
CpG 2 −0.67 0.47 0.16 −1.28 0.56 0.03 −0.65 0.28 0.02 −2.27 1.02 0.03 −0.82 0.44 0.07 −2.93 1.76 0.10
CpG 3 −0.49 0.55 0.37 −0.75 0.36 0.04 −0.76 0.33 0.02 −2.49 1.05 0.02 −0.66 0.45 0.14 −2.36 1.72 0.17
CpG 4   −1.13 0.53 0.04 −0.64 0.25 0.01 −2.61 0.97 0.01 −0.70 0.38 0.07 −3.13 1.74 0.08
CpG 5 −1.22 0.52 0.02   −2.78 1.02 0.01 −0.88 0.50 0.08 −2.63 1.67 0.12
CpG 6 −1.08 0.44 0.02 −2.85 1.07 0.01 −0.80 0.45 0.08 −2.10 1.54 0.18
CpG 7    −0.74 0.43 0.09  
CpG 8 −0.61 0.39 0.12
CpG 9 −0.75 0.43 0.09
CpG 10 −0.62 0.39 0.12
Mean −0.62 0.50 0.22 −1.10 0.47 0.02 −0.65 0.27 0.02 −2.51 1.01 0.01 −0.73 0.42 0.09 −2.66 1.67 0.12
  1. The model shown adjusts for age (as a continuous variable) and being a patient (yes/no) at the fertility clinic as possible confounding variables. Percentages for each CpG site represent the deviation of methylation percentage for overweight/obese men (BMI ≥ 25) from the methylation percentage of normal weight men (BMI < 25). These deviations were also averaged over all CpG sites associated with a particular imprinted gene (shown on the bottom line).