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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Kaiso mediates human ICR1 methylation maintenance and H19 transcriptional fine regulation

Fig. 3

Lentiviral-mediated Kaiso knockdown (Kaiso KD) is associated with a reduction of ICR1 methylation and decreased endogenous IGF2 and H19 transcription in human primary. As controls, transfections with shRNA against a scrambled locus (S.L.) were performed. a Knockdown efficiency was quantitated by RT-qPCR. The expression values are normalized to PDH and B2M transcripts and are represented as mean ± SEM from two independent experiments each. p values for the transcriptional comparisons are indicated. b ICR1 methylation was determined by pyrosequencing of the three established differentially methylated ICR1 CpGs in bisulfite-treated DNA. The location of the analysed CpGs is schematically depicted on top. Mean methylation values of three replicates are presented ± SEM for each CG position. The knockdown of Kaiso is associated with reduced methylation of endogenous ICR1

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