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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Kaiso mediates human ICR1 methylation maintenance and H19 transcriptional fine regulation

Fig. 1

Human ICR1 is bound by Kaiso. a Schematic overview of the maternal human ICR1 consisting of two clusters each containing one 450-bp (A-) repeat with OCT4/SOX2 binding sites, followed by three to four 400-bp (B-) repeats. Each B-repeat, with the exception of the shortened B4-repeat, contains a CTS (black ovals) and a ZFP57 binding site. An optimal KBS (5′TNGCAGGA3′) resides in B4 (white oval). KBS-similar motifs (5′TNGCAGGC3′) can be detected in repeats B1, B2, B3, B5 and B6. White lollipops indicate CGCG motives as putative binding sites for Kaiso when methylated. b Chromatin immunoprecipitation (ChIP) with a specific anti-Kaiso antibody from primary fibroblast cell culture extracts indicates precipitation of the B-repeat DNA (CTS containing repeats B1, B2, B3, B5 and B6) as well as ICR1-B4-DNA (B4-KBS). Binding of Kaiso to the known functional KBS in the MMP7 promoter was used as a positive control for the ChIP. Data are represented as mean ± SEM from four independent ChIP assays, and enrichment was normalized against the input. Relative DNA concentrations were analysed by qPCR indicating significant pulldowns for MMP7, CTS and B4-KBS. P values for the individual pulldowns are indicated

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