Fig. 5

Increased P21 and reduced cyclin B1 by 5-AZA and vitamin C in HCC lead to cell cycle arrest. a Quantitative changes in P21 mRNA in HLE and Huh7 treated with vitamin C, 5-AZA, and 5-AZA + vitamin C compared to the untreated control after 48 h. Data was normalized using GAPDH expression as a reference control. b Western blot analysis for changes in P21 and cyclin B1 proteins in HLE and Huh7 with various treatments. Calculations were normalized with GAPDH protein. c Graphs represent the calculation of changes in cyclin B1 protein in HLE and Huh7 with the various treatments. Data was normalized using GAPDH expression as a reference control. All the data are the average of the experiments (N = 3). Statistical significance was tested using one-way ANOVA (non-parametric) Tukey’s test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.01. Error bars represent the standard deviation