Fig. 2

5-AZA and vitamin C inhibit cell proliferation and induce cell cycle arrest in HCC. a PCNA nuclear staining of HCC cell lines, HLE and Huh7, treated with vitamin C, 5-AZA, and 5-AZA + vitamin C for 48 h. Graphs represent the calculation of the percentage of PCNA-positive cells as an indicator of inhibition of cell proliferation in HLE and Huh7. b Cell cycle analysis indicating the stage of cell cycle arrest in HLE and Huh7. All the data are the average of the experiments (N = 3). Statistical significance was tested using one-way ANOVA (non-parametric) Tukey’s test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.01. Error bars represent the standard deviation