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Table 3 Clinical sensitivity and specificity for methylation detection using overall survival as end point

From: Analysis of RET promoter CpG island methylation using methylation-specific PCR (MSP), pyrosequencing, and methylation-sensitive high-resolution melting (MS-HRM): impact on stage II colon cancer patient outcome

 

Sensitivity (%)

Specificity (%)

Direct-MSP

10.7

86.5

Nested-MSP

33.1

73.8

Pyrosequencing 5 % threshold

22.8

73.1

Pyrosequencing 10 % threshold

17.8

83.6

Pyrosequencing 15 % threshold

16.1

85.3

Pyrosequencing 20 % threshold

14.4

87.7

Pyrosequencing 25 % threshold

11.0

90.2

MS-HRM

15.4

87.7

  1. Sensitivity was calculated as a/(a + b). Specificity was calculated as d/(c + d)
  2. MSP methylation-specific PCR, MS-HRM methylation-sensitive high-resolution melting