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Table 2 Methylation frequencies of RET promoter CpG island methylation

From: Analysis of RET promoter CpG island methylation using methylation-specific PCR (MSP), pyrosequencing, and methylation-sensitive high-resolution melting (MS-HRM): impact on stage II colon cancer patient outcome

 

Direct-MSP

Nested-MSP

Pyroseq. 5 %

Pyroseq. 10 %

Pyroseq. 15 %

Pyroseq. 20 %

Pyroseq. 25 %

MS-HRM

Frequency of methylation in % (n)

12.0 (25/209)

29.6 (71/240)

25.8 (62/240)

17.1 (41/240)

15.4 (37/240)

13.3 (32/240)

10.4 (25/240)

13.8 (33/239)

HR for 3-year follow-up (95 % CI)

1.52 (0.66–3.52)

1.74 (0.97–3.15)

1.13 (0.60–2.11)

1.53 (0.78–2.97)

1.59 (0.80–3.16)

1.85 (0.93–3.86)

1.57 (0.97–3.44)

1.83 (0.92–3.65)

HR for 5-year follow-up (95 % CI)

1.13 (0.51–2.53)

1.21 (0.72–2.04)

0.89 (0.51–2.57)

1.14 (0.62–2.09)

1.20 (0.63–2.26)

1.46 (0.77–2.76)

1.34 (0.65–2.76)

1.44 (0.76–2.72)

  1. MSP methylation-specific PCR, Pyroseq. pyrosequencing, MS-HRM methylation-sensitive high-resolution melting, N number of cases, HR hazard ratio, CI confidence interval