Pyrosequencing | MSP | MS-HRM | |
---|---|---|---|
Material | FF preferred | FFPE/FF | FF preferred |
Closed-tube | √ | ||
€/sample | 4.76 | 1.21–1.64b | 1.76 |
Amplicon length (bp) | <400a/<200 bp | <160 | <200 |
Annealing temp. (°C) | 50–68 | 56a/60–66 | 58–66 |
Number of CpG dinucleotides included in primer | 1 or less/none | 1 or lessa/3 or more | 2 or less |
Analysis of CpG dinucleotides | CpG dinucleotides in between primers | CpG dinucleotides covered by primers | CpG dinucleotides covered and in between primers |