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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Analysis of RET promoter CpG island methylation using methylation-specific PCR (MSP), pyrosequencing, and methylation-sensitive high-resolution melting (MS-HRM): impact on stage II colon cancer patient outcome

Fig. 1

Schematic representation of the RET promoter CpG island (according to the EMBOSS database; located on chromosome 10q11.2) and the genomic region of primer location. Each rectangle represents a CpG dinucleotide. CpG dinucleotides, which are underlined, point to the primer locations for all four methylation analysis techniques. Direct-MSP and nested-MSP (here: MSP) share the same primers specific for methylated, as well as not methylated, DNA. Flanking primers used for nested-MSP amplify bisulfite-treated DNA, without discriminating between methylated and not methylated DNA. Therefore, these primers are not included in this figure

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Clinical Epigenetics

ISSN: 1868-7083

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