Fig. 4

Influence of NMP on T3-L1 adipogenic maturation. a Two-day post-confluent 3T3-L1 pre-adipocytes were cultured with differentiation medium (MDI) in the absence or presence of NMP (5 or 10 mM) for 10 days. b Representative micrographs showing the cell monolayers stained with Oil Red O. For the quantitative analysis, Oil Red O staining was extracted with isopropyl alcohol and the absorbance at ʎ490 nm was measured. Data are presented as mean ± SD. c Real-time PCR was used to quantify the effect of NMP on adipogenic gene expression (PPARγ). On day 8, PPARγ mRNA expression was analyzed by real-time PCR. Result is presented as ΔΔCT. *P < 0.01 MDI-treated cells vs. control; P < 0.01 NMP + MDI-treated cells vs. MDI-treated cells