Fig. 3

Influence of NMP on adipocyte differentiation from hMCS. a Human bone marrow-derived mesenchymal cells (MSCs) were induced into adipogenesis by differentiation medium (MDI) containing IBMX, dexamethasone, indomethacin, and insulin as described in the “Methods” section. After 14 days, the lipid droplets were stained with Oil Red O. For the quantitative analysis, Oil Red O staining was extracted with isopropyl alcohol and the absorbance at ʎ490 nm was measured. Data are presented as mean ± SD. b Representative micrographs showing the cell monolayers stained with Oil Red O. As seen from the staining and the quantification in cells treated with differentiation medium alone, there is much more lipid accumulation than in cells treated with both differentiation medium and NMP (5 and 10 mM). However, no significant difference was observed between the two different doses of NMP. c Real-time PCR analysis for PPARγ mRNA expression. The levels of PPARγ were normalized to the levels of PPIA, suitable reference genes during adipogenic differentiation of MSCs. Result is presented as ΔΔCT. *P < 0.01 MDI-treated cells vs. control; P < 0.05 NMP + MDI-treated cells vs. MDI-treated cells