Fig. 8
JMJD6 promotes tumor growth of cells derived from MMTV-Myc mammary gland tumors. a 106 Myc83 cells (with amplification of the chromosome 11 locus containing JMJD6) stably expressing empty vector (EV) or two independent shRNAs targeting JMJD6 were injected into mammary fat pads of FVB/N mice and tumors were measured over the next month. b 5 × 105 88CT1 cells (no chromosome 11 amplification) with stable expression of LacZ control or wild-type JMJD6 were surgically implanted into mammary fat pads and tumor growth was measured over the next 20 days. c Tumors from b were formalin fixed and sectioned and the percentage of dead cells was analyzed by TUNEL assay. d Tumors from b were immunostained with anti-Ki67 antibodies and signal intensity was quantitated by ImageJ software. e Western blot analysis of pro-survival genes in 88CT1 cells expressing JMJD6