Fig. 2

DNA methylation levels in the TREM2 TSS-associated region in Alzheimer’s disease (AD) and control hippocampus. a The figure shows the genomic position of the amplicon surveyed by bisulfite cloning sequencing at the TSS-associated region of the TREM2 gene. At the bottom of the figure, the GWAS-associated TREM2 variant (rs75932628) position is also shown. b The picture shows the methylation pattern at CpG site resolution of TREM2 TSS-associated region for different samples across AD progression. It is only an example of the bisulfite results for each group. Therefore, two individual subjects (samples) have been included per group. Black and white circles represent methylated and unmethylated cytosines, respectively. Each column symbolizes a unique CpG site in the examined amplicon, and each line represents an individual DNA clone. Global percentage of methylation for each analyzed sample (control or patient) at this particular amplicon is indicated at the bottom of each sample. Meth Amp methylation amplicon, GWAS genome-wide association studies