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Fig. 6 | Clinical Epigenetics

Fig. 6

From: Global histone modification profiling reveals the epigenomic dynamics during malignant transformation in a four-stage breast cancer model

Fig. 6

The regulation of tumorigenesis by KDM3A. a KDM3A stable knockdown cell lines were made in HMC-L and -LT. Cell viability was measured by MTT assay. b KDM3A/JMJD1A was knocked down in HMC-LT, and cell cycle was analyzed by flow cytometry with PI staining. c RTCA cell migration assay was performed as described with KDM3A/JMJD1A overexpression and knockdown cells. d KDM3A/JMJD1A was stably knocked down in T47D breast cancer cell line. Cells were seeded in soft agar and colonies were counted 4 weeks later. Experiments were repeated three times, with two duplicates in each experiment. e The protein level of KDM3A and H3K9me2 in the collected breast cancer and paired adjacent tissues is measured with western. f The mRNA levels of MYC and PAX3 in the 15 paired cancer and adjacent tissues are measured by quantitative RT-PCR. g The level of KDM3A protein, H3K9me2, MYC, and PAX3 mRNA was summarized. *P < 0.05, **P < 0.01

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