Fig. 5

KDM3A/JMJD1A regulates the transcriptional program of multiple oncogenes during transformation. a, b Expression of DEGs in KDM3A/JMJD1A-deficient cell lines and LT/p6 or LTR/p6 was shown in the heatmap. c, d Biological process analysis of DEGs which were rescued by KDM3A deficiency in transformed cells back to the level in primary cell (marked by arrows in a and b). e Venn diagrams reveal the amount of decreased H3K9me2 LOCKs in LT or LTR restored by KDM3A/JMJD1A knockdown. f KDM3A/JMJD1A knockdown reduced the mRNA level of oncogenes in HMC-LTR. g KDM3A/JMJD1A deficiency increased H3K9me2 level on MYC, BCL2, PAX, PRL, and AGR2 locus. h A cell line stably expressing F-KDM3A/JMJD1A was established from breast cancer cell line T47D. ChIP assay with anti-Flag was performed as indicated. i, j Serum was withdrawn from the medium culturing T47D cells for 24 h, and FBS was added back to induce MYC expression. KDM3A deficiency reduced MYC mRNA and protein induction. *P < 0.05, **P < 0.01