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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Global histone modification profiling reveals the epigenomic dynamics during malignant transformation in a four-stage breast cancer model

Fig. 2

Reduction in H3K9me2 and me3 levels in transformed cell lines and breast cancer tissue. a Profile of histone modifications in transformed cell lines by Western blotting. The relative level of each modification vs histone H3 was measured with ImageJ and labelled below the corresponding band. H3K9me2 and me3 levels decrease along with transformation, coupled with H3K9ac increase. b Decreased H3K9me2 and me3 in breast cancer tissues. Commercially purchased paraffin tissue chips including normal adjacent tissue (NAT) and breast cancer (CA) were stained with fluorescent antibodies. c, d Box plots displaying fluorescence densities of H3K9 me2/me3 in cancer and normal tissues. Fluorescence densities were measured by Olympus FV1000. Dots represent the ratio of H3K9 methylation/DAPI in each sample. P value <0.05. P values were calculated by Wilcoxon rank sum test

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Clinical Epigenetics

ISSN: 1868-7083

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