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Fig. 4 | Clinical Epigenetics

Fig. 4

From: Splicing-dependent expression of microRNAs of mirtron origin in human digestive and excretory system cancer cells

Fig. 4

Different splicing factors can be involved in the biogenesis of specific mirtronic miRNAs. a The amount of SRSF1 and SRSF2 proteins markedly increased in transfected HCT116 cell line. Western-blot analysis was carried out on nuclear extracts of cells transfected with empty vector pcDNA3 (-) or plasmid containing recombinant SRSF1 gene (pSRSF1) or SRSF2 gene (pSRSF2). Beta-actin was used as an internal loading control. b Splicing factor-dependent alterations of endogenous mirtronic miRNA expression levels were determined using RT-qPCR. The mean values were calculated for three (SRSF1) and five (SRSF2) biological replicates. *Statistically significant results, p < 0.05

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Clinical Epigenetics

ISSN: 1868-7083

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