Fig. 2

ROC curve for detection of hypermethylated RASSF1A. Data plot of the sensitivity and 100 % specificity. Serum DNA was isolated from serum from 24 patients with metastatic breast cancer and 24 age-matched healthy control individuals. The DNA was incubated with either the methylation-resistant restriction enzymes HpaII or BstUI and thereafter PCR amplified with six different primer designs (primer ID # 32, 40, 50, 54, 63, and 66, as explained in Table 2) covering different regions of the promoter and exon 1 of RASSF1A