Discovery of new methylation markers to improve screening for cervical intraepithelial neoplasia grade 2/3

Clinical Epigenetics

Table 7 Combinations of different methylation markers to create a panel of genes most suited as triage test in HPV-positive scrapings ranked on highest sensitivity (n = 152)

Gene combination	Sensitivity CIN2+	Specificity CIN2+	Sensitivity CIN3+	Specificity CIN3+
JAM3/CDH6	80 %	64 %	88 %	50 %
ANKRD18CP/CDH6/EPB41L3	79 %	61 %	89 %	51 %
CDH6/EPB41L3	77 %	61 %	88 %	53 %
GFRA1/EPB41L3/CDH6	78 %	61 %	88 %	53 %
ANKRD18CP/CDH6	77 %	61 %	85 %	51 %
GFRA1/ANKRD18CP/CDH6	77 %	61 %	85 %	51 %
JAM3/EPB41L3/ANKRD18CP	78 %	72 %	88 %	58 %
C13ORF18/JAM3/ANKRD18CP ^a	77 %	81 %	85 %	61 %
ANKRD18CP/EPB41L3	75 %	75 %	86 %	63 %
GFRA1/EPB41L3/ANKRD18CP	75 %	75 %	86 %	63 %
C13ORF18/CDH6	73 %	61 %	83 %	54 %
JAM3/GFRA1/ANKRD18CP ^a	76 %	81 %	85 %	63 %
C13ORF18/JAM3/EPB41L3	76 %	72 %	86 %	60 %
GFRA1/CDH6	72 %	64 %	83 %	57 %
JAM3/ANKRD18CP ^a	76 %	83 %	85 %	64 %
JAM3/EPB41L3	75 %	75 %	86 %	63 %
JAM3/EPB41L3/GFRA1	75 %	75 %	86 %	63 %
GFRA1/EPB41L3	72 %	78 %	85 %	68 %
C13ORF18/EPB41L3	72 %	75 %	85 %	65 %
C13ORF18/JAM3/GFRA1	70 %	86 %	81 %	71 %
JAM3/GFRA1	69 %	89 %	81 %	74 %
C13ORF18/ANKRD18CP	68 %	83 %	76 %	67 %
C13ORF18/JAM3	69 %	92 %	80 %	74 %
GFRA1/ANKRD18CP	67 %	83 %	76 %	68 %

^aThese gene combinations showed the highest combined sensitivity and specificity

ISSN: 1868-7083