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Fig. 1 | Clinical Epigenetics

Fig. 1

From: MSRE-HTPrimer: a high-throughput and genome-wide primer design pipeline optimized for epigenetic research

Fig. 1

The workflow of MSRE-HTPrimer pipeline. The MSRE-HTPrimer pipeline can be run via an intuitive web interface. The sequential analysis steps are displayed from top to bottom. (Single asterisk) Restriction enzyme cut sites prediction step and the Type-II restriction enzymes list input are only applicable for MSRE-PCR. (Double asterisks) Quality filter table file is optional; if user-defined primer selection criteria are not provided, then all primer pairs will be recorded in the final output summary table. (Triple asterisks) Download and prepare reference sequence and annotation from UCSC genome browser step runs only once first time or when reference genome information is changed

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