Genome- and epigenome-wide association study of hypertriglyceridemic waist in Mexican American families

Clinical Epigenetics

Table 2 Characteristics of the CpG sites significantly associated with HTGW

Characteristic	cg00574958	cg17058475	cg06500161
Chromosome	11	11	21
Coordinate	68607622	68607737	43656587
Gene symbol	CPT1A	CPT1A	ABCG1
Gene context	5’UTR	5’UTR	Body
Relation with CpG Island	North shore	North shore	South shore
Presence of SNP within probe	Yes	No	Yes
Minor allele frequency of probe SNP in cohort subset	0.0000	-	0.0279
Probe SNP ➔ Methylation association in cohort subset	-	-	Not significant
Cross-reactivity	None	None	None
Heritability	0.21	0.37	0.47
p heritability	2.7 × 10⁻⁵	3.5 × 10⁻¹⁰	2.2 × 10⁻¹⁵
Median β
HTGW	0.0831	0.0887	0.5831
No HTGW	0.0929	0.0993	0.5667
Association with HTGW^ab
b	−0.48	−0.44	0.36
p	1.7 × 10⁻¹⁵	8.7 × 10⁻¹¹	3.7 × 10⁻¹⁰
q	7.9 × 10⁻¹⁰	4.0 × 10⁻⁵	1.7 × 10⁻⁴
Specificity of association with HTGW^b
b	−0.46	−0.40	0.32
p	1.3 × 10⁻¹²	3.4 × 10⁻⁸	1.8 × 10⁻⁷
Association with HTGW and its components estimated through an interactive model
High TG and WC
b	−0.71	−0.53	0.73
P	2.3 × 10⁻⁷	0.0002	5.3 × 10^-8
High WC only
b	−0.22	−0.25	0.27
P	5.7 × 10⁻⁵	0.0815	0.0002
High TG only
b	−0.20	0.05	0.47
P	0.7969	0.2690	0.5328
Association with T2D that is mediated through HTGW^c
b	−0.16	−0.16	0.15
P	0.0223	0.0180	0.0086

b, p, and q indicate regression coefficient, nominal significance value, and significance level corrected for multiple testing (after correction for genomic inflation), respectively
^aAssociation between DNA methylation and HTGW after adjusting for age, age², sex, age × sex interaction, and age² × sex interaction, Illumina Sentrix® ID and Sentrix® position (to account for batch effects), use of anti-lipid, anti-hypertensive and anti-diabetic medications and cellular heterogeneity
^bAssociation between DNA methylation and HTGW after adjusting for age, age², sex, age × sex interaction, and age² × sex interaction, Illumina Sentrix® ID and Sentrix® position (to account for batch effects), use of anti-lipid, anti-hypertensive and anti-diabetic medications, cellular heterogeneity, presence of type 2 diabetes, obesity (body mass index ≥ 30 Kg/m²), systolic and diastolic blood pressure
^cMediation estimated using Sobel’s parameter [32]. All regression models were adjusted for age, age², sex, age × sex interaction, and age² × sex interaction, Illumina Sentrix® ID and Sentrix® position (to account for batch effects), use of anti-lipid, anti-hypertensive and anti-diabetic medications, and cellular heterogeneity

ISSN: 1868-7083