Fig. 6

Silencing of ADAMTS19 with shA19e22 reduces the migration capabilities of SW480 cells. Transwell migration assay using a matrix of collagen I. Experiments were performed in three independent replicates, using three wells per cell line in each replicate, and examining three to six microscope fields per well. No statistically significant difference was detected between replicates. In gray are boxplots combining all the values per cell line. In orange are boxplots showing the values of individual wells in controls (SW480 and SW480 transfected with shLuc) and in blue the ADAMTS19-silenced cell line SW480 transfected with shA19e22. A 35.8 % reduction of median migratory capabilities was observed in SW480 cells transfected with shA19e22. P values were calculated by Tukey’s honest significance method on a nested ANOVA model